[Product Name] Generic Name: Rapid Antibody Detection Card for Clostridium Sheep Disease (Colloidal Gold Method)
[Packaging Specification] 50T/box
[Principle] The rapid antibody detection card for clostridiasis is made using the colloidal gold immunochromatography test (GICA) principle. After adding the sample to the loading well, it moves along the chromatographic membrane together with the colloidal gold label. If Clostridium antibodies are present in the sample, they bind to the colloidal gold marker and antigen on the test line, resulting in a purplish-red color. If no Clostridium antibody is present in the sample, no color reaction occurs.
[Kit Composition]
Serial number | Name | 50 per box |
1. | Test card (including straw) | 50 units |
2. | Sample diluent | 50 copies |
[Storage and Shelf Life] Store in a cool, dry place (2-30°C), shelf life is 24 months.
[Sample Requirements]
1. This test card is only suitable for testing sheep serum/whole blood.
2. Serum: Draw 2-3ml of blood as usual and place it in a clean, dry test tube, let it stand for about 1 hour until coagulated, then centrifuge at 4000 rpm for 10 minutes (blood can also be left to stand for about 2 hours until coagulation and serum precipitates naturally), then separate the serum. The serum must be clear, without hemolysis or contamination. Serum samples can be stored at 2~8°C in the short term, but at -20°C in the long term.
3. Whole blood: Fresh anticoagulant whole blood is collected on the same day; for whole blood without anticoagulants, the rapid antibody test card for blotridiomyces is freshly collected and used.
[Test Method]
1. Serum/whole blood sample addition method: Tear open the aluminum foil packaging of the test card, take out the test card, place it on a flat, clean counter, use the pipette inside the bag to slowly drop 1 drop of serum or 2 drops of whole blood into the sample well, then add 2 drops of diluent to the loading well.
2. Leave at room temperature for 10-15 minutes to determine the result; color results after 15 minutes are invalid.
[Result Judgment]
1. Negative: In the observation hole, only a purplish-red line appears in the reference line area (C).
2. Positive: In the observation well, both the detection line area (T) and the control line area (C) show purple-red lines. The higher the antibody titer, the darker the color of the test line (T).
3. Ineffective: No purplish-red lines appear in the reference line area (C) inside the observation hole.
[Diagnostic Reference]
1. If the sheep has not been vaccinated against clotridiosis:
A. If no obvious color bands appear at the test line T of the sample, and the sample does not contain Clostridium antibodies, if the population is healthy, it means there is no Clostridium infection and Clostridium infection vaccination should be administered promptly. If acute symptoms appear, Clostridium infection cannot be ruled out.
B. If a clear color band appears at the test line T of the sample, it indicates the animal has Clostridium infection or a previous infection, which should be further verified. 2. If the sheep has been vaccinated against closcodiosis:
A. When a distinct color band appears at the test line T of the sample, it indicates that the antibody titer of Clostridium is high, reaching a level of protection against wild toxin attacks, and also indicates a low likelihood of being infected with wild toxins.
B. When no obvious color bands appear at the test line T of the sample, it indicates that antibody titers have not reached the protective titers required to resist Clostridium attack, posing a risk of infection with wild venom. Vaccination is recommended for catch-up.
[Limitations of the Test Method]
This Clostridium sheep disease antibody rapid test card is for screening only and does not serve as a basis for diagnosis.
[Notes]
1. Please read the manual carefully before testing. All reagents provided by this product are for use only.
2. Do not use other animal serum or other liquids that are not required for the sample as negative controls.
3. Products that are expired or have damaged aluminum foil bags must not be used.
4. When removing the test card from the refrigerator, it should be opened after returning to room temperature. Once opened, the test card should be used as soon as possible to avoid deterioration after moisture.
5. Avoid using contaminated, cloudy, severely hemolytic, or large blood lipid samples.
6. Avoid contact with the chromatography film at the sample holes and detection windows on the test card.
7. Waste from the test should be considered pollutants and properly handled according to local regulations.
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